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No. Anything started from ground up is a startup eventually it shapes into a business.

Earlier stages, when things are totally not defined it’s called a startup. A business is later stages. When a company doesnt need funding anymore and can self sustain most probably thru the public markets. In the core any startup is a business. If anything that has a transaction can be termed as business, so can start ups.

Start-up is the part of business so at the end when you're we'll settled startup people will call you business owner not an startup owner. Uber is also startup but now business every single thing Is startup.

Startup is mostly a business with innovative ideas, aiming for rapid growth and often operating in a highly uncertain environment. While business, on the other hand, can refer to any organization engaged in commercial, industrial, or professional activities, including startups, but it typically implies a more established and stable entity with a focus on sustainability and profitability.